TABLE 3.
Summary of PG muropeptide composition for C. jejuni 81-176, Δape1, ΔpatB, ΔpatA, Δape1C, ΔpatBC, ΔpatAC, and Δoap
| Muropeptide species | Percentage of peak areaa |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Sample Set #1 |
Sample Set #2 |
||||||||
| 81-176 | Δape1 | ΔpatB | ΔpatA | 81-176 | Δape1C | ΔpatBC | ΔpatAC | Δoap | |
| Monomers (total) | 43.5 | 49.1 | 44.9 | 41.7 | 42.2 | 41.5 | 39.6 | 41.2 | 40.0 |
| Dipeptide | 17.8 | 11.0* | 18.0 | 15.0 | 13.6 | 11.0 | 11.2 | 17.2 | 16.4 |
| Tripeptide | 6.7 | 16.4* | 8.5 | 10.2* | 10.1 | 11.1 | 9.7 | 6.6* | 6.7* |
| Tetrapeptide | 18.3 | 20.9 | 17.6 | 15.8 | 17.2 | 18.1 | 17.1 | 16.1 | 15.5 |
| Pentapeptides-Gly | 0.6 | 0.9* | 0.7 | 0.7 | 0.8 | 1.0 | 1.3* | 0.9 | 1.0 |
| O-Acetylated | 1.0 | 8.0* | 0.3* | 0.3* | 1.9 | 2.4 | 1.7 | 1.1* | 0.8* |
| Dimers (total) | 47.7 | 40.7 | 47.6 | 49.4 | 48.8 | 49.4 | 49.5 | 48.5 | 48.1 |
| TetraTri | 16.8 | 14.2 | 18.4 | 18.5 | 16.0 | 15.5 | 15.2 | 15.7 | 15.2 |
| TetraTetra | 30.5 | 25.6 | 28.6 | 29.9 | 32.0 | 33.0 | 33.2 | 32.0 | 31.7 |
| TetraPentaGly | 0.4 | 0.9* | 0.6* | 1.0* | 0.8 | 0.9 | 1.1* | 0.8 | 1.2* |
| Anhydro-Dimers | 13.5 | 8.2* | 12.7 | 12.1 | 11.8 | 11.3 | 11.6 | 12.6 | 12.4 |
| O-Acetylated | 2.2 | 4.9* | 1.7 | 0* | 3.2 | 3.7 | 2.3 | 0.4* | 0* |
| Trimers (total) | 8.8 | 10.2 | 7.5 | 8.8 | 7.8 | 8.1 | 9.5 | 8.9 | 10.2* |
| TetraTetraTri | 0.9 | 0.5* | 1.0 | 1.5* | 1.0 | 0.9 | 1.3* | 1.3* | 1.4* |
| TetraTetraTetra | 8.0 | 9.7 | 6.5 | 7.3 | 6.8 | 7.2 | 8.2 | 7.6 | 8.8 |
| Dipeptides (total) | 17.8 | 11.0* | 18.0 | 15.0 | 13.6 | 11.0 | 11.2 | 17.2 | 16.4 |
| Tripeptides (total) | 15.4 | 23.6* | 18.0 | 19.9 | 18.4 | 19.1 | 17.7 | 14.9 | 14.7 |
| Tetrapeptides (total) | 65.9 | 64.0 | 62.9 | 63.9 | 65.1 | 67.1 | 67.6 | 64.8 | 65.1 |
| Pentapeptides (total) | 0.8 | 1.3* | 1.0 | 1.2* | 1.2 | 1.5 | 1.9 | 1.3 | 1.6* |
| O-Acetylated (total) | 2.1 | 10.4* | 1.2* | 0.3* | 3.5 | 4.3 | 2.9 | 1.3* | 0.8* |
| Anhydromuropeptides | 8.3 | 4.8* | 7.8 | 7.5 | 7.7 | 7.4 | 7.9 | 8.3 | 8.6 |
| Average chain length | 12.0 | 20.8* | 12.8 | 13.3 | 13.0 | 13.5 | 12.6 | 12.0 | 11.6 |
| Degree of cross-linkage | 29.7 | 27.2 | 28.8 | 30.6 | 29.6 | 30.1 | 31.1 | 30.2 | 30.9 |
| % peptide cross-links | 56.5 | 50.9 | 55.1 | 58.3 | 57.8 | 58.5 | 60.4 | 58.8 | 60.0 |
a Values represent the percentage area of each muropeptide from raw data (found in Table 2) calculated to give a total of 100%. Boldface numbers represent a change in relative abundance of ≥20% from wild type. Boldface numbers with an asterisk represent ≥30% change from wild type. Percentages shown are calculated from values rounded to the nearest 0.1%. Muropeptide profiles were generated in two sets of experiments, Sample Set #1 and Sample Set #2. Muropeptide profiles were compared with the wild-type 81-176 muropeptide profile that was analyzed in the same sample set.