TABLE 3.
Values for Michaelis-Menten kinetic parameters and inhibition constants (Ki) for HIV-1 PR constructs
Values in parentheses indicate the relative ratio referenced to data for subtype B.
| Sample | Km | kcat | kcat/Km | Ki of SQV | Ki of APV | Ki of ATV | Ki of DRV |
|---|---|---|---|---|---|---|---|
| μm | s−1 | s−1μm−1 | nm | nm | nm | nm | |
| B | 12 ± 3 (1.0) | 7.2 ± 2.8 (1.0) | 0.60 ± 0.12 (1.0) | 3.91 ± 0.05 (1.0) | 0.72 ± 0.05 (1.0) | 0.69 ± 0.09 (1.0) | 0.25 ± 0.03 (1.0) |
| B-E35D | 13.6 ± 1.3 (0.9) | 2.8 ± 0.5 (0.4) | 0.20 ± 0.04 (0.3) | 3.6 ± 1.2 (0.9) | 0.71 ± 0.12 (1.0) | 0.83 ± 0.18 (1.2) | 0.51 ± 0.12 (2.1) |
| PR5 | 18 ± 3 (1.3) | 5.9 ± 0.8 (0.8) | 0.40 ± 0.10 (0.6) | 3.3 ± 0.9 (0.8) | 0.96 ± 0.07 (1.3) | 0.67 ± 0.05 (1.0) | 0.32 ± 0.06 (1.3) |