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. 2016 Sep 6;291(43):22757–22768. doi: 10.1074/jbc.M116.737387

FIGURE 3.

FIGURE 3.

BMXΔN stability and the N-end rule influencing apoptosis. A, PC3 cells expressing control or UBR1/2 targeting shRNAs were transfected to express either wild type BMXΔN, the valine N-terminal mutant, or a vector control. 24 h after transfection, cells were treated with 10 nm docetaxel for 48 h. The cells were then analyzed by trypan blue staining. The data represent the average and S.D. (error bars) from three independent experiments, and p values were determined from paired two-tailed t tests. B, FACS analysis of docetaxel treated PC3 cells. PC3 cells were transfected to express either wild type BMXΔN, the valine N-terminal mutant, or a vector control. Cells were then treated with the indicated concentrations of docetaxel for 48 h. Cells were then analyzed by FACS for annexin V and propidium iodine staining. The percentage of cells that were stained with either annexin V (dark gray) or doubly labeled with both annexin V and propidium iodide (gray) are indicated. The data represent the average and S.D. from three independent experiments and p values were derived from paired two-tailed t tests.