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Summary of actin-activated ATPase activity and in vitro motility results
ATPase (represented as mean ± S.D.) and in vitro motility (represented as mean ± S.E.; n = 177 filaments) were reported from at least two protein preparations and 2–3 independent experiments.
| Construct | V0 | kcat | KATPase | Velocity |
|---|---|---|---|---|
| s−1 | s−1 | μm | nm/s | |
| MYO3A 2IQ cGFP | 0.04 ± 0.05 | 1.83 ± 0.02 | 3.3 ± 0.2 | 70.62 ± 0.48 |
| MYO3B 2IQ cGFP | 0.02 ± 0.01 | 0.50 ± 0.13 | 66.8 ± 31.0 | 31.20 ± 0.38 |
