TABLE 3.
List of primers used to generate novel constructs for this study
| Gene/species (accession no.) | Plasmid (template vector) | Primers used (5′ to 3′), forward (F) and reverse (R) |
|---|---|---|
| MYO3A/human (NM_017433) | MYO3A.MtD (pEGFP-C1) | F, GCATTGGCATTCTTGATATATTTGCCTTTGAAAATTTCAAAAAAAATT |
| R, AATTTTTTTTGAAATTTTCAAAGGCAAATATATCAAGAATGCCAATGC | ||
| MYO3A.ΔTHD2 (pEGFP-C1) | F, AGAGAGCCAGCAGCCGGCGGCCGCAACCCCTACGACTTC | |
| R, GAAGTCGTAGGGGTTGCGGCCGCCGGCTGCTGGCTCTCT | ||
| MYO3A 2IQ (pFastBac) | F, TCGGGCGCGGATCCCATGGTAGATGATTTAGC | |
| R, GCTAAATCATCTACCATGGGATCCGCGCCCGA | ||
| MYO3B.3A Tail (pEGFP-C1) | F, TTTCGTGAAGAAACAAGCAGAAAATGGATCCTCTGCTAATGAAAGATTCATTTCAG | |
| R, CTGAAATGAATCTTTCATTAGCAGAGGATCCATTTTCTGCTTGTTTCTTCACGAAA | ||
| MYO3B/human (NM_138995) | MYO3BKIN (pEGFP-C1) | F, TAGGATCCAAACATCTGTATGGA |
| R, TAGCGGCCGCTTAATGTTGAGCAAAAGAGTC | ||
| MYO3B (pEGFP-C1) | F, TAGGATCCAAACATCTGTATGGA | |
| R, TAGGATCCGATGATTTGGTCAACCTAGA | ||
| MYO3B 2IQ (pFastBac) | F, TAGGATCCATGGATGATTTGGTCAACCTAGA | |
| R, TAGCGGCCGCGTCCCCTGCTTGATT | ||
| MYO3B.3A Tail (pEGFP-C1) | F, AGTGCCGAGGTTCAAGGATCCAGCGAGCCTGGTGAC | |
| R, GTCACCAGGCTCGCTGGATCCTTGAACCTCGGCACT |