FIGURE 1.

Neurotoxicity of Tat-immunodepleted supernatants from Tat-expressing astrocytes. U373.MG cells were transfected with pcDNA3 or pTat.Myc. Culture supernatants were harvested 72 h post-transfection and incubated with anti-Myc antibody for Tat immunodepletion (ID/α-Myc). Normal rabbit IgG was used as the control (ID/IgG). In addition, the supernatant without immunodepletion (No ID) was also included as a control. All supernatants were exposed to LTR-Luc-transfected 293T for their activity on the LTR promoter (A) or exposed to SH-SY5Y and evaluated for their effects on cell viability (B). Error bars, S.D.; *, p < 0.05.