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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Radiat Res. 2016 Sep 19;186(4):385–395. doi: 10.1667/RR14463.1

Figure 3. Simultaneous inhibition of Trx- and GSH-metabolism enhances radiation responses.

Figure 3

(A) Normalized clonogenic survival is shown for MDA-MB-231 (black bars) and SUM159 cells (gray bars). 2-AAPA treatment for 3 hours followed by 2 Gy IR. * p<0.05 compared to control, δ p<0.05 compared to treatment groups alone. (B) PANC-1 cells were treated 24 hours with 100 units/ml PEG-CAT or PEG alone, 200 µM SSZ and 500 nM Au for the last 3 hours of incubation followed by 2 Gy IR. Errors ± 1 SEM of at least 3 separate experiments. * p<0.05 vs control, ** p<0.05 vs treatments alone and α - p<0.05 vs treatment without PEG-CAT. (C, D, E) MDA-MB-231 cells were treated with 100 µM BSO for 24 hours and/or 500 nM Au for 1 hour followed by 1,2,4,6 and 8 Gy IR immediately followed by the clonogenic survival assay. Results were normalized to treatment alone and the resulting graph analyzed using IGOR Pro 6.36. (F) Normalized clonogenic survival is shown for MDA-MB-231 (black bars) and SUM159 cells (gray bars). 100 µM BSO for 24 hours +Au (250 nM SUM159 or 500 nM MDA-MB-231) for the last hour, followed by 2 Gy IR. * p<0.05 compared to control, δ p<0.05 compared to treatment groups alone.