Table 2.

Application of peroxidase-based approaches to proximity-dependent protein labeling

Applications	Methods	Targeting locations	Conjugated molecules	References
Horse radish peroxidase	EMARS	Lipid raft	Glycosylphosphatidylinositol (GPI) targeting motifs	[36]
			anti-β1 integrin monoclonal antibody, Cholera toxin subunit B (CTxB)	[35]
			anti-ganglioside GD3 monoclonal antibody	[85]
			anti-β1 integrin monoclonal antibody, anti-EGFR monoclonal antibody, anti-IGF1R monoclonal antibody, anti-EphA2 monoclonal antibody, CTxB anti-CD3ε antibody	[32]
		Cell surface molecules associated with Thy1	anti- Thy1 monoclonal antibody	[86]
		Cell surface molecules associated with β1 integrin	anti-β1 integrin monoclonal antibody	[87]
		Cell surface molecules associated with CD20	Rituximab, anti-CD20 monoclonal antibody	[88]
		Proteins associated with PrPC	Anti-PrPC antibody	[89]
	SPLAAT	BRC cluster at the membrane	anti-chicken IgM (target receptor BCR is a IgM)	[37]
Engineered- ascorbate peroxidase	APEX	Proteomics of mammalian cell mitochondria matrix	Mitochondria matrix targeting motif	[34]
		Proteomics in D. melanogaster mitochondria	Mitochondria matrix targeting motif	[44]
		Proteomics of mammalian mitochondrial intermembrane space	Mitochondria intermembrane targeting sequence	[46]
		Proteomics of mammalian cilia	N-terminal cilia targeting sequence of NPHP3	[42]
	APEX2	Ultrastructural localization of ER and mitochondria proteins	NES (6–17 residues of HIV1 Rev protein), MICU1, mitochondria outer membrane targeting motif (C-terminal 31 residues of MAVS), ER targeting motif (N-terminal 27 residues of rabbit p450)	[41]
		Ultrastructural localization of ER and mitochondria proteins	ER targeting motif, Tom20, H2B, HMOX1, TRMT61B, MGST3, Sco1, Sec61B, AURKAIP1, BIT1/PTRH2, COX14, MRPL12, MRM1, MRPS15, PLGRKT, QIL1/MIC13, SFXN1, SVH/ARMC10, TRMT2B, Pdk1	[90]
		Proteomics of mammalian mitochondrial matrix and intermembrane space	Mitochondria matrix and intermembrane targeting motifs	[45]
		Yeast proteomics	Golgi	[91]
		Mammalian ER-PM junctions	STIM1	[43]