A, The activity of 100μM inhibitors (labeled 1–4 for compounds 1–4), or the control with 0.5% DMSO (labeled D) incubated with sGC and with varying concentrations of BAY 58-2667. B, The inhibition activity of 100μM compounds 1, 2, or 0.5% DMSO (labeled D) incubated with sGC and indicated concentrations of BAY 41-2272 in the absence of NO donor. C, The inhibitory activity of 100μM compounds 1, 2, or 0.5% DMSO (labeled D) incubated with sGC and varying concentrations of BAY 41-2272 in the presence of 1μM SNAP. The error bars represent SEM; the rate measurements were performed in triplicate.