Figure 6. Effect of β-Lap on NQO1 activation and NAD+/NADH ratio, evaluated in vitro and in vivo. (A, B) Expression of NQO1 protein and assay for NQO1 enzymatic activation, and role of β-Lap and dicumarol from cytosolic fraction of A549 cell line. (C) Effect of β-Lap on intracellular NAD+/NADH ratio in A549 cells. (D) Effect of β-Lap on NAD+/NADH ratio in BLM-treated lung tissues of mice. NAD+/NADH ratio was analyzed following manufacture's instruction. *p<0.05 by one-way analysis of variance compared with the control, BLM, and β-Lap. β-Lap: β-lapachone; NQO1: NADH:quinone oxidoreductas; BLM: bleomycin.
