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. 2016 May 17;7(22):31602–31622. doi: 10.18632/oncotarget.9395

Figure 1. Elevated expression of ROCK1, ROCK2 and RhoA in malignant T4-2 cells in three-dimensional laminin-rich ECM (3D lrECM).

Figure 1

A. mRNA expression of ROCK1 and ROCK2 in S1 and T4-2 cells monolayer (2D) and 3D lrECM culture were analyzed by real-time quantitative Reverse Transcription PCR (RT-PCR) with specific primer sets. mRNA expression level of ROCK1 and ROCK2 were normalized to that of TATA binding protein (TBP). Values represent means ± SE of six experiments. ROCK1; N.S. (not significant), **P < 0.01, ***P < 0.001 compared with S1 2D group (Student's t). ROCK2; N.S. (not significant), ***P < 0.001 compared with S1 2D group (Student's t). The Illustration of morphologies of S1 and T4-2 cells in 2D and 3D is shown in the bottom. B. Protein expression of ROCK1, ROCK2, EGF receptor (EGFR), Integrinβ1, RhoA, phosphorylated myosin light chain (P-MLC), E-cadherin and Lamin A/C in nonmalignant S1 cells and malignant T4-2 cells in 2D and 3D lrECM cultures. Total cell lysates were analyzed by Western blotting with their specific antibodies.