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. 2016 May 17;7(22):31602–31622. doi: 10.18632/oncotarget.9395

Figure 9. Involvement of ROCK in disorganized phenotype of MDA-MB-231 cells in 3D lrECM.

Figure 9

A. Morphological alternations of MDA-MB-231 cells treated with ROCK inhibitor (Y-26732 or Fasudil, 30 μM). Scale bars: 20 μm. B. Partial inhibition of proliferation of MDA-MB-231 cells treated with ROCK inhibitor (Y-26732 or Fasudil, 30 μM), EGFR inhibitor (AG1478, 0.1 μM) and MEK inhibitor (PD98059, 10 μM). Cell viability was assessed by MTT assay. Values represent means ± SE of four experiments. *P < 0.05, ***P < 0.001 compared with vehicle control group (Student's t). C. Protein expression of ROCK1, ROCK2, EGFR, Integrinβ1, GLUT3, RhoA, P-MLC and Lamin A/C in MDA-MB-231 cells. Total cell lysates were analyzed by Western blotting with their specific antibodies. D. Confocal immunofluorescence images of MDA-MB-231 cells treated with ROCK inhibitor (Y-26732 or Fasudil, 30 μM). Their images were obtained using specific antibody or Alexa Fluor-conjugated phalloidin (Integrinα6; Green, F-actin; Red, Nuclei; Blue). Scale bars: 10 μm.