Figure 1. miR-7-5p inhibits melanoma cell proliferation in vitro.
A. Cell viability (MTS) assay of WM266-4, A2058, 1205Lu and SK-MEL-2 cells transfected with miR-7-5p or miR-NC precursor molecules for 5 d at 5 nM. *, p-value < 0.01. B. Representative images of 2D (plastic) and 3D (Matrigel) colony formation assay of WM266-4 cells transfected with miR-7-5p or miR-NC precursor molecules for 14 d period (30 nM) and stained with crystal violet. Scale bar = 200 μm. Colonies were counted manually with ImageJ software. *, p-value < 0.001. C. Flow cytometric cell cycle analysis of WM266-4 cells transfected with miR-7-5p or miR-NC precursor molecules for 3 d at 30 nM.