Table 1. RT-PCR analysis of liver gene expression following PHx in the Gal1-KO compared to WT mice.
Genes | Hours post-PHx | Il6-induced | Inflam-mation | Lipid meta-bolism | Other | ||||
---|---|---|---|---|---|---|---|---|---|
Gene symbol | Gene ID NCBI | Alternative name | 2 | 6 | 24 | 2 h # | |||
Acly | 104112 | Atpcl | − | down | NC | V | |||
Atf3 | 11910 | Lrf1 | down | NC | NC | V | |||
Ccl3 | 20302 | Mip1a | − | NC | down | V | |||
Ccna1 | 12427 | Cyclin A | − | − | NC | V | |||
Ccr1 | 12768 | Rantes-R | − | − | down | V | |||
Cd14 | 12475 | down | down | down | V | V | |||
Cd36 | 12491 | Fat | − | − | NC* | V | V | ||
Cidec | 14311 | Fsp27 | − | NC | down | V | |||
Crem | 12916 | Icer | NC | NC | − | V | |||
Egr1 | 13653 | Ngfi-A | NC | NC | NC | V | V | ||
Fabp4 | 11770 | Afabp | − | down | down | V | |||
Flt1 | 14254 | Vegfr1 | − | − | down* | V | |||
Fos | 14281 | c-Fos | NC | NC | − | V | V | ||
Gadd45a | 13197 | Ddit1 | NC | NC | NC | V | V | ||
Hgf | 15234 | Sf | − | − | NC* | V | |||
Hif1a | 15251 | Mop1 | − | − | NC* | V | |||
Igfbp1 | 16006 | Ibp1 | NC | NC | − | V | V | ||
Il6 | 16193 | Ifnb2 | down | NC | NC | V | |||
Jun | 16476 | Ap-1c | down | NC | NC | V | V | ||
Kdr | 16542 | Vegfr2, Flk1 | − | − | NC* | V | |||
Lgals1 | 16852 | Galectin-1 | ND | ND | ND* | V | |||
Lgals3 | 16854 | Galectin-3 | − | NC | NC | V | |||
Lta | 16992 | Lymphotoxin α | ND | ND | - | V | |||
Ltb | 16994 | Lymphotoxin β | NC | NC | NC | V | |||
Ltbr | 17000 | Lymphotoxin β receptor | down | down | NC | V | V | ||
Map2k4 | 26398 | Jnkk1, Mek4 | Up | NC | NC | V | V | ||
Myc | 17869 | c-Myc | Up | Up | NC | V | V | ||
Plau | 18792 | u-PA | NC | Up | − | V | V | ||
Plin4 | 57435 | S3-12 | − | down | down | V | |||
Ppara | 19013 | Ppar, Nr1c1 | NC | NC | NC | V | |||
Saa1 | 20208 | Saa-1 | − | NC | NC | V | |||
Socs3 | 12702 | Cis3 | − | NC | NC | V | |||
Tgfb1 | 21803 | Tgf-beta1 | NC | NC | down* | V | |||
Thpo | 21832 | Thrombopoietin | down | NC | − | V | V | ||
Tnf | 21926 | Tnf-alpha | NC | NC | down | V | |||
Tnfaip3 | 21929 | A20 | NC | Up | down | V | V | ||
Tnfsf14 | 50930 | Light, Hveml | NC | NC | NC | V | |||
Zfp36 | 22695 | Tristetraprolin | NC | Up | Up | V | V |
NC - not changed (comparable expression in WT and Gal1-KO post-PHx livers)
ND - not detected; Up – up-regulated; down – down-regulated; − not tested
tested by semi-qRT-PCR; others were tested by qRT-PCR
“Up” or “down” at least two-fold in Gal1-KO compared to WT post-PHx liver is shown in bold
genes whose induction at 2 h post-PHx was IL6-dependent [Li, 2001 #1370].