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. 2016 Apr 28;7(22):31772–31789. doi: 10.18632/oncotarget.9091

Figure 1. The capacity of zymosan-stimulated neutrophils to induce trans-PMVEC protein leak is inhibited by subanesthetic isoflurane.

Figure 1

A. Neutrophils were sequentially treated with zymosan (ZY) for 15 min and 0.7% isoflurane (ISO) for 15 min where indicated, followed by incubation with zymosan for 12 h. Treatment of cells with solvent serves as control groups for zymosan and isoflurane. The media were collected and added to the monolayer PMVEC. Trans-PMVEC EB-albumin leak was determined. B. Neutrophils were treated as described in (A), followed by measurement of NO production and ONOO− release by neutrophils. C. Neutrophils were treated with zymosan or solvent for 12 h, followed by treatment with or without FeTPPS (25 mM) or SIN-1 (200 mM) for 15 min. The media were replaced with fresh complete media, which were collected 6 h later and added to the monolayer PMVEC. Trans-PMVEC EB-albumin leak was determined. Data are represented as the mean ± SEM of 3 replicates. *P < 0.05, #P < 0.01, as compared with zymosan group.