Skip to main content
. 2016 Apr 28;7(22):31772–31789. doi: 10.18632/oncotarget.9091

Figure 5. Zymosan activates c-Src through TLR2 signaling in neutrophils.

Figure 5

A.-C. Neutrophils were transfected with indicated siRNAs prior to treatment with or without zymosan for 6 h. Cells were then subjected to Western blot analysis (A, C) and measurement of NO and ONOO− production (B). D. Neutrophils were treated with zymosan (10 μg/ml) for indicated time periods. Cell lysates were prepared and immunoprecipitated using c-Src antibody, followed by Western blot analysis of the precipitated proteins. Data are represented as the mean ± SEM of 3 replicates or representative of 3 independent experiments. #P < 0.01, as compared with the cells exposed to scrambled siRNA alone [A (upper panel)]. *P < 0.05, #P < 0.01, as compared with the cells exposed to zymosan alone (B, C), zymosan + scrambled siRNA [A (lower panel), B, C], or vehicle alone (D).