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. 2016 Apr 28;7(22):31772–31789. doi: 10.18632/oncotarget.9091

Figure 6. Isoflurane decreases c-Src activity by targeting the NMDA glutamate receptor.

Figure 6

A. Neutrophils were treated with zymosan (10 μg/ml) for 6 h in the presense of the NMDA receptor agonist NMDA (50 mM) or antagonist MK-801 (30 mM) where indicated. Cell lysates were then subjected to Western blot analysis. B., C. Neutrophils were transfected with indicated siRNAs prior to treatment with or without zymosan (10 μg/ml). Cells were then subjected to Western blot analysis 6 h later (B) and measurement of NO and ONOO− production 12 h later (C). D. Neutrophils were pretransfected with indicated siRNAs, and/or treated with zymosan (10 μg/ml) for 12 h in the presence of the indicated reagents including NAC (50 mM), MK-801 (30 mM) and BAPTA (25 mM). The media were replaced with fresh complete media, which were collected 6 h later and added to the monolayer PMVEC. Trans-PMVEC EB-albumin leak was determined. Data are represented as the mean ± SEM of 3 replicates or representative of 3 independent experiments. *P < 0.05, as compared with the cells exposed to zymosan alone (A). *P < 0.05, #P < 0.01, as compared with the cells exposed to zymosan + scrambled siRNA (B-D).