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. 2015 Dec 19;7(22):31814–31824. doi: 10.18632/oncotarget.6674

Figure 2. Biotransformation of PPL7 by endophyte YA09 (Delftia acidovorans).

Figure 2

(A) PPL7 was biotransformed into two new compounds during culture with YA09. The culture medium after biotransformation at 30°C for 5 days was spotted on a TLC plate and separated by solvent mixture (chloroform: methanol = 3:1). Lane 1: culture medium with PPL7, without YA09 but incubated at 30°C for 5 days; lane 2: reference material of PPL7; 3 μg/ml PPL7 was added to the culture medium; lane 3: culture medium after biotransformation of PPL7 by YA09. Yellow arrows, new compounds (lower, ZH-1; upper, ZH-2); red arrow, PPL7; lane 4: culture medium with YA09 and PPL7 before biotransformation; lane 5: culture medium with YA09 and without PPL7. Histogram: statistical analysis of Rf values for PPL7, ZH-,1 and ZH-2 in (A) and (B). (B) Products of biotransformation were separated by column chromatography to obtain ZH-1 and ZH-2. Lane 1: culture medium after biotransformation of PPL7 by YA09; lane 2: culture medium before biotransformation; lanes 3 and 4: chromatography-purified ZH-1; lanes 5 and 6: chromatography-purified ZH-2. (C-E) ESI-MS data and chemical structures of PPL7 (C), ZH-1 (D), and ZH-2 (E).