Skip to main content
. 2016 Feb 3;7(22):31832–31846. doi: 10.18632/oncotarget.7149

Figure 1. Identification of novel miRNAs that regulate c-FLIPL expression in renal cancer cells.

Figure 1

A. MiR-708 binding sites in the 3′UTR of c-FLIPL mRNA. B. Quantitative RT-PCR analysis of relative miR-708 expression levels in human renal cancer cell lines (A498, Caki, and ACHN) and a normal immortalized renal cell line (HK2) (Upper panel). Relative c-FLIPL expression in human renal cancer cell lines and a normal renal cell line (Bottom panel). C. RT-PCR analysis of c-FLIPL mRNA in Caki cells transfected as indicated (Upper panel). Immunoblots for c-FLIPL in Caki cells transfected as indicated. Actin was used as the loading control (Bottom panel). D. Immunoblots for c-FLIPL in Caki cells transfected as indicated.