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. 2016 Feb 3;7(22):31832–31846. doi: 10.18632/oncotarget.7149

Figure 2. The c-FLIPL isoform is a functional target of miR-708 in renal cancer.

Figure 2

A. Schematic representation of c-FLIPL showing putative miR-708 target sites (Left panel). Also shown are the nucleotides mutated in c-FLIPL 3′UTR mutant (Right panel). B. Luciferase activity assay with the respective wild-type luciferase constructs containing two different miR-708 target sequences (site #1 or site #2) transfected with miR-cont or miR-708. For all luciferase activity assays, firefly luciferase values were normalized to renilla luciferase activity and plotted as the relative luciferase activity. The data is reported as the mean ± SD (n = 3). Statistics, Student's t-test for unpaired values. * indicates P < 0.05 versus miR-cont-transfected cells. C. Luciferase assays showing repression of the wild-type UTR (c-FLIPL 3′-UTR) or mutant UTR (c-FLIPL 3′UTR-mutant) following transfection of miR-708, miR-708 mimics, anti-miR-708, and miR-cont. The data is reported as the mean ± SD (n = 3). * indicates P < 0.05 versus miR-cont-transfected cells.