Figure 5. Downregulation of c-FLIPL mediated by si-cFLIPL enhanced sensitivity to various apoptotic stimuli.
Caki cells were transfected with miR-708 or si-c-FLIPL with miR-Cont or si-Cont. Twenty-four hours after transfection, the cells were treated with TRAIL A. TG B. and Dox C. for 24 h. Apoptosis was analyzed as the sub-G1 fraction by FACS (Left panel). aP < 0.05 compared to miR-Cont-transfected cells treated with the indicated drugs. bP < 0.05 compared to si-Cont-transfected cells treated with the indicated drugs. Immunoblots for c-FLIPL and actin (Right panel).