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. 2016 Feb 3;7(22):31832–31846. doi: 10.18632/oncotarget.7149

Figure 6. Restoration of miR-708 directly mediated downregulation of c-FLIP expression in vitro and increased the sensitivity of renal cancer cells to various apoptotic stimuli.

Figure 6

A. Quantitative RT-PCR analysis of the relative miR-708 expression levels in stably transfected A498 or Caki cells to confirm overexpression of miR-708 in the pooled cells. B. RT-PCR analysis and Western blotting to analyze the relative c-FLIPL expression levels in stably transfected A498 or Caki cells. C. Caki/miR-cont and Caki/miR-708 cells were treated with the indicated drugs for 24 h. The cellular DNA content was measured after propidium iodide staining. The proportion of apoptotic cells is indicated. The data is reported as the mean ± SD (n = 3). *P < 0.05 versus Caki/miR-708 cells treated with the indicated drugs.