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. 2016 Feb 17;7(22):31892–31906. doi: 10.18632/oncotarget.7441

Figure 4. mTORC1 is required for Akt induction of NF-κB in HNSCC.

Figure 4

A. Overexpression of Akt increases activity of mTORC1 and NF-κB. Different doses of active HA tagged Akt2 were transfected in Cal27 cells and phosphorylation p65 and S6K and total p65 and S6K, as well as HA-Akt2 expression were tested by western blot. B. Inhibition of Akt, mTORC1 and NF-κB by Akt inhibitor, perifosine. Cells were treated with different doses of perifosine for 24 hours and the levels of p65, Akt, S6K and of endogenous phosphorylation of S6K, p65, and Akt were determined by probing with the indicated antibodies. C. Depletion of Raptor blocks Akt induction of NF-κB. Cells were transfected with non-target siRNA or siRNA against Raptor as indicated for 48 hours and active Akt2 were transfected for another 24 hours. Cells were lysed and the levels of p65, Akt, S6K and of endogenous phosphorylation of S6K, p65, Akt2, and Raptor were determined. The results are representative of three experimental repetitions.