Figure 5. EGFR^vIII affects viability of DK-MG cell lines.

A. Comparison of viability of both DK-MG cell lines, measured by propidium iodide incorporation after 3 days of culturing under the indicated conditions. B. Cleavage of PARP for both cell lines was assessed using western blotting. Full length and cleaved forms of PARP are indicated on the left hand side of panels. C. Representative images showing activity of the synthetic Caspase 3/7 reporter in serum free media (top panel) or supplemented with 20 ng/mL EGF (bottom panel). The number of reporter positive cells was higher in the DK-MG^low line under both culture conditions, without any signs of clumping of apoptotic cells. 40x objective used. Error bars indicate SEM. Statistical significance calculated by paired Student's t-test. *, p < 0.05; **, p < 0.01; ***, p < 0.005.