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. 2016 Apr 1;7(22):32088–32099. doi: 10.18632/oncotarget.8525

Figure 3. TR4 knockdown led to weakened chemosensitivity of Huh7 and Hep3B cells.

Figure 3

(A) qPCR and Western blot analysis results showing successful TR4 knockdown in Huh7 cells. Huh7 were infected with lentivirus carrying either sh-TR4 or scrambled (scr) control sequence, and TR4 mRNA and protein levels were analyzed by qPCR and Western blot analysis, respectively. GAPDH served as a control in analyses. (B, C) drug sensitivity test for cisplatin in Huh7-shTR4 and Huh7-scr cells. Cells were treated with various indicated concentrations of Line 5 should read drugs for 48 h (left panel) or treated with 4 μg/ml cisplatin (right panel) and analyzed every 24 h for 3 days, cell viability upon drug treatment was analyzed by an MTS assay. (D) qPCR and Western blot analysis results showing successful TR4 knockdown in Hep3B cells were infected as in (A) and TR4 mRNA and protein levels were analyzed by qPCR and Western blot analysis, respectively. GAPDH served as a control in analyses. (E, F) drug sensitivity test for cisplatin in Hep3B-shTR4 and Hep3B-scr cells. Cells were treated as in (B–C) for 48 h or treated with 6 μg/ml cisplatin and analyzed as in (B–C). All assays were performed in triplicate (*P < 0.05, **P < 0.01, ***P < 0.001).