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. 2016 Apr 1;7(22):32088–32099. doi: 10.18632/oncotarget.8525

Figure 4. TR4 overexpression led to enhanced chemosensitivity of LM3 and SNU387 cells.

Figure 4

(A) qPCR and Western blot analysis results showing successful TR4 overexpression in LM3cells. LM3 were infected with lentivirus carrying either wpiTR4 or wpi control sequence, and TR4 mRNA and protein levels were analyzed by qPCR and Western blot analysis, respectively. GAPDH served as a control in analyses. (B, C) drug sensitivity test for cisplatin in LM3-wpiTR4 and LM3-wpi cells. Cells were treated with various indicated concentrations of cispaltin for 48 h or treated with 12 μg/ml cislatin and analyzed every 24 h for 3 days, cell viability upon drug treatment was analyzed by an MTS assay. (D) qPCR and Western blot analysis results showing successful TR4 overexpression in SNU387 cells. SNU387 were infected as in (A) and analyzed by qPCR and Western blot analysis. GAPDH served as a control in analyses. (E, F) drug sensitivity test for cisplatin in SNU387-wpiTR4 and SNU387-wpi cells. Cells were treated with various indicated concentrations of cisplatin for 48 h or treated with 18 μg/ml cisplatin and analyzed every 24 h for 3 days, cell viability upon drug treatment was analyzed by an MTS assay. All assays were performed in triplicate (*P < 0.05, **P < 0.01, ***P < 0.001).