Figure 7. Pol ι promotes ESCC cell migration and invasion through the JNK-AP1 pathway.

Pol ι overexpression cells were pretreated with SP600125 for 24h. A. western blot analysis of the expression levels of JNK, p-JNK, MMP-2 and MMP-9. B. wound-healing assay at 0, 48 and 96h after scratch. The open wound area was normalized to the area at the initial time (0 h, *p < 0.05, **p<0.01, Student's t test). C. cells were plated in matrigel transwell inserts and incubated for 48h. Cells migrated to the bottom were stained with crystal violet and calculated manually (**p<0.01, Student's t test).