A. Western blotting analysis of the expression levels of GRK2, CXCR2, phosphorylated AKT, ORF65 and vIL-6 in BC3 cells transduced with lentivirus-mediated a mixture of short hairpin RNA targeting GRK2 (shGRK2) or the vector control (mpCDH) and stimulated with TPA for 48 h. B. Real-time DNA-PCR was used to detect the viral genome copy number in the supernatants of BC3 and BCBL-1 cells treated as in (A). *P < 0.05 and **P < 0.01 for Student's t-test. C. The mRNA expression levels of ORF21, ORF57, ORF26, ORF59, ORF-K9 and RTA in BC3 and BCBL-1 cells treated as in (A) were quantitated by RT-qPCR. *P < 0.05, **P < 0.01 and *** P < 0.001 for Student's t-test. D. Western blotting analysis of the expression levels of CXCR2, phosphorylated AKT, RTA, ORF65 and vIL-6 in BC3 cells transduced with lentivirus-GRK2 (GRK2) or the control (pHAGE). The antibody against His-tag was used to detect the exogenous expression of GRK2. E. Real-time DNA-PCR was used to detect the viral genome copy number in the supernatants of BC3 and BCBL-1 cells treated as in (D). *P < 0.05 and ***P < 0.001 for Student's t-test. F. The mRNA expression levels of ORF21, ORF57, ORF26, ORF59, ORF-K9 and RTA in BC3 and BCBL-1 cells treated as in (D) were quantitated by RT-qPCR. *P < 0.05, **P < 0.01 and ***P < 0.001 for Student's t-test.