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. 2016 Apr 5;7(22):32286–32305. doi: 10.18632/oncotarget.8591

Figure 6. Inhibition of CXCR2/AKT pathway disrupts KSHV latency and suppresses angiogenesis.

Figure 6

A. Western blotting analysis of the expression levels of CXCR2, phosphorylated AKT, RTA and vIL-6 in BC3 cells transduced with lentivirus-mediated a mixture of short hairpin RNAs targeting CXCR2 (shCXCR2) or the control (mpCDH). B. Real-time DNA-PCR was performed to detect the viral genome copy number in the supernatants of BC3 and BCBL-1 cells treated as in (A). ** P < 0.01 for Student's t-test. C. BC3 cells transduced with lentivirus-mediated a mixture of short hairpin RNAs targeting CXCR2 (shCXCR2) or the control (mpCDH) were examined for pro-angiogenic effects in nude mice by the Matrigel plug assay. The hemoglobin level of the Matrigel plugs was determined with O.D. value at 540 nm. Data represented mean ± SD, each group with five tumors (n=5). Three independent experiments were performed and similar results were obtained. *P < 0.05 for Student's t-test. D. The mRNA expression levels of HIF1α, HIF2α, VEGFR2 and MMP13 were measured using RT-qPCR in tumor tissues from the Matrigel plugs treated as in (C). **P < 0.01 and ***P < 0.001 for Student's t-test. E. Western blotting analysis of phosphorylated AKT and vIL-6 in BC3 cells transduced with lentivirus-mediated a mixture of short hairpin RNAs targeting AKT (shAKT) or the control (mpCDH). F. Real-time DNA-PCR was performed to detect the viral genome copy number in the supernatant of BC3 cells treated as in (E). ** P < 0.01 and *** P < 0.001 for Student's t-test. G. The Matrigel plug assay was performed to examine the pro-angiogenic effect of BC3 cells transduced with shAKT (shAKT) or the control (mpCDH) in nude mice. The hemoglobin level of the Matrigel plugs was determined with O.D. value at 540 nm. Data represented mean ± SD, each group with five tumors (n=5). Three independent experiments were performed and similar results were obtained. *P < 0.05 for Student's t-test. H. RT-qPCR was performed to detect the mRNA expression levels of HIF1α, HIF2α, VEGFR2 and MMP13 in tumor tissues from the Matrigel plugs treated as in (G). *P < 0.05 and **P < 0.01 for Student's t-test.