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. 2016 Apr 5;7(22):32286–32305. doi: 10.18632/oncotarget.8591

Figure 7. Deletion of miR-K3 from the KSHV genome reactivates KSHV lytic replication but inhibits angiogenesis.

Figure 7

A. Matrigel assay analysis of microtubule formation was performed in EA.hy926 cells, which were incubated with the supernatants from stable iSLK-BAC16 (KSHV_WT) or BAC16ΔmiR-K3 (miR-K3_Mut) cells, as described in the ‘Materials and Methods’ section. The photographs of microtubules were taken at 8 and 16 h post-seeding (original magnification, × 100). B. Quantification of results in (A). Data represented mean ± SD. ***P < 0.001 for Student's t-test. C. The mRNA expression levels of KSHV ORF59, ORF-K9 and RTA in HUVECs infected with BAC16 KSHV wild type virus (KSHV_WT) or BAC16 miR-K3 deletion mutant virus (miR-K3_Mut) were detected by RT-qPCR. *P < 0.05 and ** P < 0.01 for Student's t-test. D. Western blotting analysis of GRK2, CXCR2, phosphorylated AKT and RTA proteins in HUVECs transduced with lentivirus-miR-K3 (miR-K3), lentivirus-mediated shGRK2 (shGRK2) or their respective control (mpCDH) after infection with BAC16 KSHV miR-K3 deletion mutant virus. E. The mRNA expression levels of ORF57, ORF26, ORF-K9 and RTA in HUVEC treated as in (D; left panel) were determined by RT-qPCR. *P < 0.05, **P < 0.01 and ***P < 0.001 for Student's t-test. F. The mRNA expression levels of ORF21, ORF57, ORF26, ORF59, ORF-K9 and RTA in HUVEC treated as in (D; right panel) were determined by RT-qPCR. *P < 0.05, **P < 0.01 and ***P < 0.001 for Student's t-test.