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. 2016 Apr 12;7(22):32362–32374. doi: 10.18632/oncotarget.8708

Figure 4. The miR-184 promoter activity is dependent on p53 status.

Figure 4

A wild-type p53 or different mutant p53 expression vectors (5 μg) and the miR-184 promoter reporter plasmid were transfected into H1299 and H358 cells and the then cells lysates were separated by SDS-PAGE for the evaluating p53 expression by western blotting using a specific antibody. Luciferase reporter assay was performed to evaluate the reporter activity of miR-184 promoter (−839/+1). The miR-184 level was determined by real-time PCR.