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. 2016 Apr 12;7(22):32362–32374. doi: 10.18632/oncotarget.8708

Figure 6. Increase in Bcl-2 expression by reduced miR-184 may be responsible for cisplatin resistance via apoptotic pathway.

Figure 6

TL-1 cells were treated with miR-184 mimic (m, 40 nM) and/or Bcl-2 expression vectors. TL-10 cells were treated with miR- 184 inhibitor (miR-184i, 40 nM) and/or shBcl-2 for 24 h. Indicated cells were treated with 0.1% DMSO or 5 μM cisplatin for 24 h. The cells were subjected to annexin V and PI staining by a flow cytometry analysis. Percentage of apoptotic cells including with the Annexin V+/PI- population (early apoptosis) plus Annexin V+/PI− (late apoptosis/secondary necrosis) was summarized by a flow cytometric analysis. Data are expressed as means ± s.d. (n = 3).