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. 2016 Apr 13;7(22):32421–32432. doi: 10.18632/oncotarget.8718

Figure 3. miR-1207-5p inhibits the AKT and STAT3 signalings.

Figure 3

(A–B) A549 cells were transfected with either miR-1207-5p mimics or control mimics for 48 hrs, and the cellular proteins and RNAs were isolated. The cellular protein levels of p-STAT3, p-AKT, p-S6 were assayed by Western blotting (A). The STAT3 signaling downstream target genes (IL-6, CCL2, CXCL-10, CCL5, IL-10, IL-11, IL-1B) mRNA expression levels were assayed by RT-qPCR (B). (C) To assay the effect of miR-1207-5p on macrophage characters, d-THP1 cells were transfected with either miR-1207-5p mimics or control mimics for 48 hrs, then, total RNA was isolated and RT-qPCR was performed to assay the M1/M2 macrophage phenotype-related genes expression levels (left); and IL-10 and IL-12/23 protein levels from cell supernatant were assayed by ELISA (right). All data are shown as the mean ± s.e.m. *p < 0.05, **p < 0.01 compared with control.