Figure 2. PAX8 expression in MOSE cells increases the FOXM1 pathway.

(A) mRNA levels (log₁₀ fold change) of genes in the FOXM1 pathway are significantly increased after PAX8 expression in MOSE cells. Data was normalized to MOSE-Neo control expression levels (n = 4). (B) Protein levels of genes in the FOXM1 pathway are increased after PAX8 expression in MOSE. (C) Western blot confirming a decrease in the FOXM1 pathway after MOSE-PAX8 transfection with FOXM1^shRNA. (D) MOSE-PAX8 cells with knockdown of FOXM1 were significantly less migratory over a wound after 2 days (n = 3) when compared to MOSE-PAX8 cells. (E) MOSE-PAX8^shFOXM1 have decreased mRNA levels (log₁₀ fold change) of EMT markers (n = 4). Data represent mean ± SD. Significance is represented by * for p ≤ 0.05.