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. 2016 Apr 16;7(22):32876–32892. doi: 10.18632/oncotarget.8765

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Copyright: © 2016 Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. The differences of cell morphology among the Hep-2 cells transfected with shRNA-mock or shRNA snail1 plasmids and control using inverted phase contrast microscope. The green fluorescence indicated that plasmids had been successfully transfected into Hep-2 cells using fluorescence microscope. B. Snail1 and N-cadherin expression levels following inhibition of Snail1 in Hep-2 cells analyzed by western blotting and RT-PCR. C. FSS induced expression of Snail, E-cad, N-cad, Twist and Vimentin in Sh-Snail Hep-2 cells. D. Wound scratch assay revealed that the cell migration distance in shRNA Snail1 was significantly shorter than that of control group after 24h.