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. 2016 Apr 16;7(22):32876–32892. doi: 10.18632/oncotarget.8765

Table 2. List of antibodies used in this study.

Category Primary antibody name Isotype Blocking Conditions 1-hr room temperature Primary Ab incubation Manufacturer
E-cad/N-Cad E-cadherin (H-108): sc-7870 rabbit pAb mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution Santa Cruz, Inc
N-cadherin (H-4):sc-271386 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:100 dilution
Tight Junction components Occludin (E-5): sc-133256 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution Santa Cruz, Inc
Claudin-5 (H-52):sc-28670 rabbit pAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution
ZO-1 (H-300):sc-10804 rabbit pAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:100 dilution
Integrins α2integrins (C-9):sc-74466 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution Santa Cruz, Inc
α5 integrins (A-11):sc-166665 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution
αV (H-2):sc-376156 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:100 dilution
β1 (A-4):sc-374429 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution
β3 (B-7):sc-46655 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution
ILK/PI3K/(p) AKT ILK (65.1):sc-20019 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution Santa Cruz, Inc
PI3K C2β (16L9):sc-100407 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:100 dilution
AKT1 (B-1):sc-5298 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution
p AKT1 (Thr 308):sc-135650 rabbit pAb 3% BSA in PBS Overnight at 4°C, 1:200 dilution
Vimentin (V9): sc-6260 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution Santa Cruz, Inc
Snail (H-130): sc-28199 rabbit pAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:100 dilution
Slug (A-7): sc-166476 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution
Twist (Twist2C1a): sc-81417 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:100 dilution
β-catenin β-catenin (E-5): sc-7963 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:100 dilution Santa Cruz, Inc
FA components Talin (8D4):sc-59881 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution Santa Cruz, Inc
Vinculin (G-11):sc-55465 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution
α-actinin (H-2):sc-17829 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution
Zyxin (H-200):sc-15338 rabbit pAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:100 dilution
FAK FAK (A-17):sc-557 rabbit pAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution Santa Cruz, Inc
Rho GTPases Rac1 (C-11):sc-95 rabbit pAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution Santa Cruz, Inc
RhoA (26C4):sc-418 mouse mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:200 dilution
Cdc42 ab64533 rabbit mAb 5% nonfat dry milk in PBS Overnight at 4°C, 1:100 dilution Abcam®, Inc