Figure 4. RT-PCR analysis of FACS-purified subfractions from the CD45⁺34⁺ cell compartment.

The subfractions numbered 0–4 contain all cells below the corresponding horizontal line. Thus, each superior subfraction also nests all previous subfractions and provides their partial repetition. Subfraction no. 5 and progenitor subfraction contain only cells from the indicated box. Subfraction no. 1 represents CD45⁺ 34⁺ 38⁻ SCs gated according to fluorescence minus one control for the CD38 antigen. Subfraction no. 0 represents even stricter gating and was isolated only if possible. Individual subfractions 0–5 were sorted and analyzed 1–2×, always with the same cell number and identical results from both analyses. Tables show the number of FACS-purified cells per one analysis and the BCR-ABL1 status of each subfraction: (−) – negative, (+) – positive, n – number of, Prog. – more mature progenitor cells, Pt. no. – patient number.