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. 2016 Apr 21;7(22):33165–33178. doi: 10.18632/oncotarget.8890

Figure 3. Effect of ACTN4 knockdown using shRNA of ACTN4 on in vitro proliferation, cdc42 activation and adherence to HUVEC.

Figure 3

A. Western blot analysis of ACTN4 expression in A549 cells infected with ACTN4 (sh#1 or sh#2) or control (shC) shRNA. B, C. Representative photographs of scanning electron microscopic analysis of A549 cells that were infected with control shRNA (B) or ACTN4 (C). Bars, 10 μm. D. Proliferation of the shRNA-infected A549 cells. E. Adherence of the A549 cells to HUVEC. F, G. Activation assay of Cdc42 in A549 cells infected with ACTN4 or control shRNAs. (F) Western blot analysis of Cdc42 in the total lysate of A549 cells. Beta-actin was blotted as a loading control. (G) Signal intensity of the active, Cdc42-GTP fraction in the A549 cells.