Figure 5. Botulinum type C protease is cytotoxic following intracellular delivery.

A. SDS gel showing botulinum type C protease and the indicated translation-inhibiting enzymes. B. Graph showing ten-fold higher vulnerability of differentiated SiMa cells to the botulinum protease (EC50=0.5 nM) in comparison to common translation-inhibiting enzymes. All enzymes were delivered into differentiated SiMa cells using LF3000 lipofection reagent (n=3, mean± SEM). Cell counts were normalised to botulinum-untreated controls (LF3000 alone).