Figure 6. Differentiated SH-SY5Y neuroblastoma cells exhibit sensitivity to botulinum type C protease.
A. The botulinum protease, introduced into cells by lipofection, causes cell death only in retinoic acid-differentiated cells. B. Graph showing dose-dependent cytotoxic effect of BoNT/C, before and after differentiation, normalised to botulinum-untreated controls (EC50=1 nM, n=3, mean± SEM). C. Immunoblots showing botulinum protease-induced SNARE cleavage and drop in SNAP23 content upon neuronal differentiation of SH-SY5Y cells.