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. 2016 Oct 24;17(1):99. doi: 10.1186/s10194-016-0694-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Effect of NTG injection on Nrf2 protein levels in the total and nuclear fractions of rat TNC a Representative immunoblots of TNC lysates. Total Nrf2 levels b and nuclear Nrf2 levels c were elevated as early as 0.5 h and persisted for 4 h after NTG injection. β-actin was used as a loading control for total Nrf2. Fibrillarin was used to assess the purity of the nuclear fraction. Data are presented as relative density units normalized to β-actin or Fibrillarin, and expressed as mean ± SD (*P < 0.01 vs the control group, # P < 0.01 vs NTG 0 h group, n = 6 per group)