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. 2016 Oct 25;6:140. doi: 10.3389/fcimb.2016.00140

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Copyright © 2016 Feng, Tan, Dai, Li, Xie, Li, Shi and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ALV-J strain identification by IFA and western blot analysis. The two ALV-J strains isolated from late and fast feathering chicken groups were detected by IFA and Western blot. (A) DF-1 cells infected with M180 strain isolated from late-feathering chicken and K243 strain isolated from fast-feathering chicken showing ALV-J specific green fluorescence, 150x magnification. (B) DF-1 cells infected with M180 and K243 produced specific ALV-J envelope protein blots. Uninfected DF-1 cells were used as a negative control (NC).