TABLE 3.

Overview of discordant molecular results submitted to resolution testing, double false-negative molecular results, and molecular detection of additional microorganisms in 10 definite infective endocarditis patients

Patient	Valve culture result	Blood culture result	PCR-ESI-MS result	16S rRNA PCR result	Definite microbiological cause
1	S. mitis, Staphylococcus hominis,a and Corynebacterium spp.a	S. mitis	Enterococcus spp.	S. mitisb/S. mitise	S. mitis
2	Negative	S. epidermidisa (two bottles out of 40 BC sets)	Viridans Streptococcus spp. and P. acnesc	Negativeb,d/S. mitise	viridans Streptococcus spp.
3	Negative	Negative	P. acnes	Negativeb,d/Negatived,e	Unknownf
4	Negative	Negative	Negatived	Negatived	Unknownf
5	Negative	Negative	Negatived	Negatived	Unknownf
6	Negative	S. epidermidis	Negatived	Negatived	S. epidermidis
7	Negative	S. mitis and Streptococcus parasanguinis	Negatived	Negatived	Viridans Streptococcus spp.
8	Negative	Streptococcus constellatus	viridans or anginosus Streptococcus spp.	S. constellatus and P. micrac	S. constellatus
9	Negative	S. epidermidis	S. epidermidis and S. capraec	S. epidermidis	S. epidermidis
10	Negative	Staphylococcus capitis	S. capitis and S. saccharolyticusc	S. capitis	S. capitis

^aThis result was considered to be contamination.

^bThis was a discordant molecular result.

^cDetection of additional microorganisms by one molecular method occurred.

^dThis was considered a false negative molecular result.

^eThis was the result of resolution testing after one freeze-thaw cycle.

^fDefinite infective endocarditis was confirmed by histologic examination of the valve.