Fig. 3.

MiR-137 directly targets EFNB2 gene and SNP rs550067317(C) independently reverses the inhibition. A. Schematic diagram of three different constructs. The arrow marked the minor allele C in the construct. B, C. Dual luciferase reporter assay of miR-137 mimics/negative co-transfected with full mutant and wild type construct in HEK293T cells after 24 h and 48 h. D. Dual luciferase reporter assay of miR-137 mimics/negative co-transfected with full mutant, rs55006731(C), and wild type construct in HEK293T cells. Bars represent SEM from three experiments.

NC, negative control. ** indicates P < 0.01, *** indicates P < 0.001.