Table 1.

Primer information for genes and small RNAs.

Gene name	Accession number	Primer	Location for the first nucleotide of the primera	Sequence (from 5′ to 3′)	Annealing temperature (°C)
EFNB2	NM_004093	Forward for RT-qPCR	Chr13:106,494,962	GATCCAACAAGACGTCCAGAA	60
		Reverse for RT-qPCR	Chr13:106,493,342	CTGAAGCAATCCCTGCAAATA
		3′-UTR Forward for mutationb	Chr13:106,492,317	CGAGCTCAGTGGGGCTGGGGAAAGGGCT	58
		3′-UTR Reverse for mutationb	Chr13:106,491,818	GCTCTAGATGACCTTTGGCTTTTCAAC
		rs550067317(C) Forward for mutation	Chr13:106,491,988	TACTGGCACTAAGATACACAGCTCCGAGCT	58
		rs550067317(C) Reverse for mutation	Chr13:106,491,972	GTATCTTAGTGCCAGTACCACAACAGTCCT
GAPDH	NM_002046	Forward for RT-qPCR	Chr12:6,537,347	CCAAGGTCATCCATGACAACT	60
		Reverse for RT-qPCR	Chr12:6,537,683	CAGGGATGATGTTCTGGAGAG
Hsa-miR-137	NR_029679	Forward for RT-qPCR	Chr1:98,046,113	TTATTGCTTAAGAATACGCGTAG	60
U6 snRNA	NR_004394	Forward for RT-qPCR	Chr15:67,840,042	CTCGCTTCGGCAGCACA	60
		Reverse for RT-qPCR	Chr15:67,839,949	AACGCTTCACGAATTTGCGT

^aUCSC Browser, Dec. 2013; http://genome,ucsc.edu/cgi-bin/hgGateway.

^bSeveral nucleotides (restriction enzyme cutting site) have been added to the 5′ terminal of the primer.