Fig. 1.

CD4mc fail to enhance recognition of HIV-1-infected cells by anti-cluster A antibodies.

Primary CD4 + T cells, which were either mock-infected or infected with NL4.3 GFP expressing the primary R5 ADA Env (NL4.3 ADA GFP), were stained with sera from 10 HIV-1-infected individuals followed by an anti-human Alexa-Fluor 647 (AF647)-conjugated secondary antibody (a, c) or with (b, d) AF647-conjugated anti-cluster A (b, d) A32, (e) N5-i5 or (f) C11, in the presence of CD4mc JP-III-48, BNM-III-170, M48U1 or DMSO (vehicle). Shown are histograms depicting representative staining obtained with (a) HIV + sera or (b) A32-AF647 and (c-f) the mean fluorescence intensities (MFI) obtained from at least three experiments. Error bars indicate mean ± SEM. Statistical significance was tested using a Kruskal-Wallis with a Dunn's post-test (**p < 0.01, ***p < 0.001, ns: non-significant).