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. 2016 Sep 9;12:208–218. doi: 10.1016/j.ebiom.2016.09.004

Fig. 3.

Fig. 3

Co-receptor binding site Fab fragments reduce HIV + sera ability to enhance recognition of infected cells in the presence of CD4mc.

(a–c) Cell-surface staining of primary CD4 + T cells either mock-infected or infected with CH58 T/F with AF647 anti-cluster A antibodies (a–b) A32 or (c) C11, in the presence or not of HIV + sera, with or without the Fab fragment of (a) 17b or (b) N12-i2 and CD4mc JP-III-48 or DMSO (vehicle). Shown in (a–c) are (left) histograms depicting representative staining and (right) the mean fluorescence intensities (MFI) obtained for staining obtained in at least 5 experiments in the presence of 10 different HIV + sera. (d) Cell-surface staining of primary CD4 + T cells isolated from 3 HIV-1-infected individuals after ex-vivo expansion with AF647-A32 in the presence or not of autologous (circle), an HIV + heterologous (square) sera, with or without the 17b Fab fragment and CD4mc JP-III-48 or DMSO. Left panels show histograms depicting representative staining with autologous sera. Right panels present the mean fluorescence intensities (MFI) obtained for multiple staining. Error bars indicate mean ± SEM. Statistical significance was tested using a Kruskal-Wallis with a Dunn's post-test (*p < 0.05, ***p < 0.001, ****p < 0.0001, ns: non-significant).