Fig. 5.

CD4-mimetic sensitize HIV-1-infected cells to ADCC-mediated by anti-cluster A antibodies in the presence of CoRBS antibodies.

Primary CD4 T cells infected with CH58 T/F were used as target cells and autologous PBMC as effector cells in our FACS-based ADCC assay. Shown in (a) are the percentages of ADCC-mediated killing obtained with serial dilution of A32 (0.3125, 0.625, and 1.25 μg/ml) alone or in combination with 17b (5 μg/ml), in the presence of CD4mc JP-III-48 or equivalent volume of DMSO for 7 different experiments. Shown in (b) are the percentages of ADCC-mediated killing obtained with A32 (0.3125 μg/ml), 17b, 17b Fab, alone or in combination, or with sera from an HIV + individual, in the presence of JP-III-48 or equivalent volume of DMSO. Error bars indicate mean ± SEM. Statistical significance was tested using an Ordinary one-way ANOVA with a Holm-Sidak's post-test (***p < 0.001, ****p < 0.0001, ns: non-significant), See also Fig. S5 and S6.