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. 2016 Sep 10;12:86–97. doi: 10.1016/j.ebiom.2016.09.007

Fig. 2.

Fig. 2

PDGFRα expression decreases TTF1, but not Pax8, expression and nuclear localization. (a) Western blot of cytoplasmic and nuclear protein extracts of the papillary thyroid carcinoma cell lines examined in this study. (b) 2D electrophoresis pattern of TTF1 as measured in BCPAP mock and PDGFRα expressing cells. The spot intensity for the two most prominent protein spots (relative to the single spot to the far left in PDGFRα expressing cells) is shown as mean ± SEM of four independent runs. (c) Confocal microscopy reveals cytoplasmic shift of TTF1 localization through comparison of BCPAP mock and BCPAP transduced with inducible PDGFRα. Scale bar 25 μm. (d) Confocal microscopy was used to quantify the change in the nuclear:cytoplasmic ratio of TTF1 localization through comparison of BCPAP mock and BCPAP transduced with inducible PDGFRα. (e) Expression of PDGFRα protein does not have a significant impact on Pax8 localization as shown by confocal microscopy. Scale bar 25 μm. (f) Confocal microscopy reveals no change in the nuclear:cytoplasmic ratio of Pax8 comparing BCPAP mock and BCPAP PDGFRα cells. (g) PTC primary cultures with and without metastases demonstrate the cytoplasmic localization of TTF1 with PDGFRα expression and this is quantified in (h). Scale bar 25 μm. Data are presented as mean ± SEM, n = 10–25 individual cells.