Fig. 3.

(A) Image of microarray showing hybridization of target amplicons generated from DNA automatically isolated and PCR amplified from Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Trichomonas vaginalis (TV). The sample preparation and PCR amplification was automatically performed using the cartridge by adding 200 μl of cultured targets (1 × 10³copies/ml) to the sample reservoir either individually or all three mixed together. The PCR amplicons were detected using the two step hybridization method described in Materials and Methods. Each universal probe was immobilized at two concentrations (20 μM, 2 μM as described in Materials and Methods). (B) To estimate the limit of detection, the target microorganisms were diluted to 25, 50 and 75 copies per ml and 200 μl of the specimens added to the cartridge either individually or all three combined. The location and concentration of the various STI target probes as well as the Spotting Controls (SC) on the DNA array are shown in the filter key.